| Flow Cytometry Calibration Particles |
| The ACME™ Calibration Particles are designed for routine calibration of flow cytometers. By using these particles, the sensitivity and linearity of the system can be determined. The sensitivity of the instrument will help detect any dye or light contamination. The linearity of the instrument will determine if the logarithmic amps and PMTs are functioning properly. |
| ACME™ Rainbow Calibration Particles |
| - Contains a mixture of fluorophores that enable the Rainbow Calibration Particles to be excited at any wavelength from 365 nm to 650 nm |
| - Allows the calibration of the FITC, PE, PE-TR, PE-Cy5, and APC channels in the flow cytometer with the same set of particles |
| - Consists of set of several similar size particles with different fluorescence intensities in a convenient dropper bottle |
| - Can be stored in the freezer and reused later since they can withstand repeated freezing and thawing |
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| Catalog No. | Description | Concentration | Nominal Size | Package Size |
| RA024-5 | Rainbow Calibration Particles, 4 peaks | 107/mL | 1.8-2.2 µm | 5 mL |
| RA036-5 | Rainbow Calibration Particles, 6 peaks | 107/mL | 3.0-3.4 µm | 5 mL |
| RA038-5 | Rainbow Calibration Particles, 8 peaks | 107/mL | 3.0-3.4 µm | 5 mL |
| RA032-5 | Rainbow Calibration Particles, 6 peaks | 107/mL | 3.2 (+/- 0.1) µm | 5 mL |
| RA0357-5 | Rainbow Calibration Particles, 4 peaks | 107/mL | 3.5-4.0 µm | 5 mL |
| RA06-5 | Rainbow Calibration Particles, 6 peaks | 107/mL | 6.0-6.4 µm | 5 mL |
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| ACME™ Ultra Rainbow Calibration Particles |
| - Designed for routine calibration of the flow cytometer in all channels |
| - Contain a mixture of fluorophores that enable the optimization of linearity, sensitivity, and resolution of the flow cytometer |
| - Especially useful in the Far Red and Violet channels |
| Catalog No. | Description | Concentration | Nominal Size | Package Size |
| URA35-2X | Ultra Rainbow Calibration Kit, 6 peaks
(Blank, 2x106/mL), (Fluorescent Particles, 107/mL) | 107/mL after mixing | 3.5-3.9 µm | 1x3 mL
1x2 mL |
| URA5-2X | Ultra Rainbow Calibration Kit, 6 peaks
(Blank 2x106/mL), (Fluorescent Particles 107/mL) | 107/mL after mixing | 5.0-5.9 µm | 1x3 mL
1x2 mL |
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| ACME™ Spectral Matching Calibration Particle Kits |
| - Surface labeled polystyrene particles with commonly used fluorophores |
| - Used to determine the linearity of the logarithmic amps and sensitivity of flow cytometers in specific channels |
| - Supplied as individual products for use in specific channels of flow cytometers |
| Catalog No. | Description | Concentration | Nominal Size | Package Size |
| EFF3-5X | FITC Easy Calibration Kit, 5 peaks | 2x106/mL | 3.0-3.4 µm | 5x1 mL |
| EF3F-5X | PE Easy Calibration Kit, 5 peaks | 2x106/mL | 3.0-3.4 µm | 5x1 mL |
| EF3-5X | PE-Cy5 Easy Calibration Kit, 5 peaks | 2x106/mL | 3.0-3.4 µm | 5x1 mL |
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| ACME™ Rainbow Linear Calibration Particles |
| Catalog No. | Description | Concentration | Nominal Size | Package Size |
| RAC03-5 | Rainbow Linear Calibration Particles (For use in Linear Scale) | 107/mL | 3.0-3.4 µm | 5 mL |
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| ACME™ ACP Calibration Particles |
| Catalog No. | Description | Concentration | Nominal Size | Package Size |
| AC03-5X | Allophycocyanin Calibration Particles, 6 peaks | 107/mL | 3.0-3.4 µm | 2x5 mL |
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| ACME™ Yellow Calibration Particles |
| Catalog No. | Description | Concentration | Nominal Size | Package Size |
| YC7-5 | Yellow Calibration Particles | 107/mL | 6.0-8.0 µm | 5 mL |
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| Flow Cytometry Calibration Particles |
| The ACME™ Rainbow Calibration Kit and Rainbow QC Kit are designed to simplify the routine calibration of flow cytometers. |
- A blank calibration particle for threshold determination
- A single intensity Rainbow Fluorescent Particle for checking the alignment and system settings of flow cytometers
- A multiple intensity mixture of Rainbow Calibration Particles for sensitivity, resolution, and linearity determination |
| Catalog No: RAC-4X ; Rainbow Calibration Kit, Consists of one vial each of the following particles: |
| Includes: | Description | Concentration | Nominal Size | Package Size |
| BCP62-2 | Blank Calibration Particles | 107/mL | 6.0-6.4 µm | 2 mL |
| RFP62-2 | Rainbow Fluorescent Particles | 107/mL | 6.0-6.4 µm | 2 mL |
| RCP32-2 | Rainbow Calibration Particles (Peaks 1-4) | 107/mL | 3.0-3.4 µm | 2 mL |
| RCP37-2 | Rainbow Calibration Particles (Peaks 5-8) | 107/mL | 3.5-4.0 µm | 2 mL |
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| ACME™ Rainbow Calibration Kits |
- Contains three multiple intensity peaks of the 8 peak Rainbow Calibration Particles set
- Used for sensitivity, resolution, and linearity determination of flow cytometers |
| Catalog No: RA03-3X; Rainbow QC Kit, Consists of one vial each of the following particles: |
| Includes: | Description | Concentration | Nominal Size | Package Size |
| RA321-5 | Rainbow Calibration Particles (Peak 1) | 107/mL | 3.0-3.4 µm | 5 mL |
| RA324-5 | Rainbow Calibration Particles (Peak 4) | 107/mL | 3.0-3.4 µm | 5 mL |
| RA328-5 | Rainbow Calibration Particles (Peak 8) | 107/mL | 3.0-3.4 µm | 5 mL |
